Ctd110.6 antibody

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August undefined, 2024

WebApr 18, 2014 · We show that CTD110.6, #3, and #10 antibodies can be used to detect cell surface glycoproteins bearing O-GlcNAc. Cell surface glycoproteins recognized by … WebO-GlcNAc Antibody (CTD110.6) is an IgM κ mouse monoclonal O-GlcNAc antibody (also designated O-linked β-N-acetylglucosamine antibody, O-β-GlcNAc antibody, O-linked …

O-GlcNAc (CTD110.6) Mouse mAb Cell Signaling …

WebClone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). chill extreme thc

CD46 Polyclonal Antibody (PA5-35311) - Thermo Fisher Scientific

WebJun 15, 2001 · The antibody reacts with a large number of proteins from cytoplasmic and nuclear extracts and readily detects in vivo changes in O-GlcNAc modification. These … WebNov 22, 2024 · Results indicate Cdcp1 (CD318) as a ligand of T-cell differentiation antigen CD6 (CD6) and a potential target for the diagnosis and treatment of … WebJan 1, 2015 · The specificity of the CTD110.6 antibody immunoreactivity was confirmed by a hapten inhibition test using 0.1 m GlcNAc. B, the levels of O-GlcNAc modification on N1EGF1–36-MycHis quantitated by densitometric analyses of immunoblots represented in A (n = 3; results are shown as mean ± S.D.; *, p < 0.01; **, p < 0.05; unpaired Student's t … chill expeditions

Alexa Fluor 594 anti-O-GlcNAc Antibody anti-O-GlcNAc - CTD110.6 …

Anti-O-GlcNAc (CTD110.6) antibody Monoclonal Antibodies

WebApr 24, 2024 · Among these antibodies, CTD110.6 and RL2 are the most widely used. However, due to a certain degree of amino acid sequence dependency, these antibodies cannot detect all proteins with O-GlcNAcylation. Additionally, a few antibodies for site-specific protein O-GlcNAcylation have been reported, ... WebApr 19, 2011 · Results. When T24 human bladder cancer cells were subjected to glucose deprivation, the expression of proteins detected by the O-GlcNAc specific antibody … grace fleisher dancing astronautWebWestern blotting of SDS-PAGE gels with O-GlcNAc-specific antibodies (RL2 and CTD110.6) shows that platelets contain 15-20 major O-GlcNAcylated proteins. Upon stimulation by agonists ( e.g. thrombin, TRAP, collagen, U46619, and A23187), the extent of modification displays protein- and agonist-specific changes. Treatment of platelets with … chilleweni cold storage

"WebThe antibody CTD110.6 is often used to detect changes in the O-GlcNAc modification. Recently, it has been demonstrated that CTD110.6 recognizes N-linked N,N'-diacetylchitobiose, which is thought ... " - Ctd110.6 antibody

Ctd110.6 antibody

WebMonoclonal Antibody for studying Ser/Thr O-GlcNAc. Cited in 70 publications. Validated for Western Blotting. Highly specific and … http://bb-china.net/search.html?brand=42&pcategory=0&classd=0&use=0&kw=&promotions=2&inventory=2&category=17&page=21

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WebResults: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized. WebCTD110.6 : Host: Mouse : Isotype: IgM : Immunogen: Synthetic peptide containing serine-O-GlcNAc. Species reactivity: Species independent : Specificity: Recognizes Ser-O-GlcNAc and Thr-O-GlcNAc in eukaryotes. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Crossreactivity:

WebO-GlcNAc (CTD110.6) is a mouse monoclonal antibody raised against a peptide containing serine-O-linked N-acetylglucosamine. PRODUCT Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of WebMay 18, 2024 · 3. Incubate the blots with CTD110.6 (diluted to 1 μg/ml in antibody dilution buffer), in duplicate, with and without 100 mM GlcNAc, overnight at 4°C. An antibody that has been antigen purified using a GlcNAc-agarose column yields higher-quality blots. Commercially available CTD110.6 is purified by ammonium sulfate precipitation.

WebO-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation. PLoS One. 2011 04 19;6(4):e18959. Zachara, Natasha E; Molina, Henrik; Wong, Ker Yi; Pandey, Akhilesh; Hart, Gerald W. The dynamic stress-induced "O-GlcNAc-ome" highlights functions for O-GlcNAc in regulating DNA … WebIn 2008, the Okajima group originally discovered the O-GlcNAc modification on Drosophila Notch EGF20 by detecting O-glycans on the ECD of Drosophila Notch using an antibody that recognizes O-GlcNAc (CTD110.6) . Later, O-GlcNAc modifications of the EGF repeats in Drosophila Delta, Serrate, and Dumpy were subsequently discovered [86,87].

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WebTarget Information. CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b … chill exo englishWebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 … grace flatwareWebCTD110.6 : Host: Mouse : Isotype: IgM : Immunogen: Synthetic peptide containing serine-O-GlcNAc. Species reactivity: Species independent : Specificity: Recognizes Ser-O-GlcNAc … chill every night clubWebApr 18, 2014 · Binding by antibodies CTD110.6, #3, and #10 was inhibited by 5 m m GlcNAc, but not 5 m m GalNAc, and was thus specific for recognition of O-GlcNAc. However, binding of antibodies 10D8, RL2, and #14 was not inhibited by the presence of 5 m m GlcNAc. Therefore, only antibodies CTD110.6, #3, and #10 should be used for cell … grace flightWebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 cells) and cells from BALB/c mice immunized with a synthetic peptide YSPTS(O-GlcNAc)PSK conjugated to KLH.This antibody recognizes O-GlcNAc in β-O-glycosidic … chillex sound reverbnationWebTime course studies by western blot with the CTD110.6 antibody demonstrated that i.v. injection in rat at 50 mg/kg increased global protein O-GlcNAcylation in total brain homogenates, starting at 1 h and peaking at 10 h and decreased pS396 tau/Tau-5 starting at 1 h, peaking at 4 h, and restoring to normal at 16 h (Yuzwa et al., 2008). chill everydayWebJul 23, 2024 · O-GlcNAc Antibody (CTD110.6) is a mouse monoclonal IgM κ O-GlcNAc antibody, cited in 62 publications, provided at 200 µg/ml; raised against O-GlcNAc … chill fabric collection by moda