WebUsing the GLAST-CreER T2 line [19,43], Robins et al. showed that GLAST-expressing tanycytes, so-called α2, that line the dorso-medial part of 3V appear first after genetic recombination that labels them and their cell progeny, … WebGLAST-GDNF-/-cKO mice also had lower densities of Brdu+ and Ki67+ cells in the PIR and exhibited larger behavioral deficits than the Ctrl mice. Mechanistically, GDNF …
Singular Adult Neural Stem Cells Do Not Exist - National Center for ...
WebApr 12, 2024 · Cre ERT2 is a recombinant fusion protein of the Cre DNA recombinase and a mutant ligand-binding domain of the human estrogen receptor. Similarly to the endogenous estrogen receptor, Cre ERT2 is... WebJun 4, 2024 · Increased Cre activity in GLAST‐CreER T2 mice after FIS. Fluorescent images of immunostaining of Cre and GFAP in the non‐ischemic (a,b) and ischemic (c,d) … try soft diet
Efficient stimulation of retinal regeneration from ... - Cell Reports
Web012586 STOCK Tg(Slc1a3-cre/ERT)1Nat/J This BAC transgenic line expresses CreERT under the control of the Slc1a3 (solute carrier family 1 (glial high affinity glutamate transporter); GLAST) promoter. When crossed with a strain containing a loxP site … WebOct 19, 2024 · We treated adult mice with the Glast-CreER:LNL-tTA:teto-Atoh1-GFP with tamoxifen for 4 consecutive days to induce Atoh1 expression in the MG; we then injured the retina with intravitreal NMDA injection, followed by the HDAC inhibitor TSA as described in our previous studies (Figure 2B). Surprisingly, we recovered almost no GFP+ cells from … WebApr 24, 2024 · Glast-EMTB-GFP transgenic mice and Glast-CreER T2 knockin mice 54 were kindly provided by E. S. Anton (University of North Carolina School of Medicine) and M. Götz (Munich Center for ... phillip raymond fulcher