Phleomycin plates
Webb29 dec. 2008 · After mixing, regenerated protoplasts were spread on square plates containing 40 ml of solidified MM. Regeneration medium contained no antibiotic, 25 μg of phleomycin ml −1, or 10 μg of nourseothricin ml −1. Selection plates were incubated at 30°C for 5 days. Isolation of genomic DNA and RNA. Webb21 jan. 2003 · The population of strains from phleomycin plates displayed a significant increase in mean lovastatin yield (P < 0.001 in a bootstrap confidence interval for …
Phleomycin plates
Did you know?
WebbStore plates and unused medium at +4°C in the dark. Plates and medium containing Zeocin™ are stable for 1-2 weeks. Recommendation Invitrogen™ imMedia™ is available … WebbPhleomycin, supplied by BioShop, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions …
WebbThe ble marker . The ble gene originates from the tallysomycin-producing actinomycetes species Streptoalloteichus hindustanus and encodes a small (13.7 kDa) protein … Webb1 maj 1996 · agar plates in the absence of phleomycin for more than. 18 months and retain resistance to the drug at 2 l g/ml. DNA was isolated from the six Pm R transformants …
WebbTo isolate a homokaryotic strain, we perform multiple subcultures of the single colonies which contain heterokaryotic cells on selection plates with hygromycin B or phleomycin to enrich for the mutated nuclei. Homokaryotic mutants can be obtained after three to four subcultures of isolated colonies. Webb25 okt. 2013 · Most commercial preparations of phleomycin suggest using 10 μg/mL for selection of S. cerevisiae on YPD, as determined by Gatignol et al. . However, we …
WebbThe phleomycin-resistant cells ( Fig. 9.6) were obtained in an average of 1/1.6 plates through selection for 3–4 weeks, with a transformation efficiency of approximately 5.5 × …
WebbIntroduction Zeocin™ is a formulation of phleomycin D1, a basic, water-soluble, copper-chelated glycopeptide isolated from Streptomyces verticillus and shows strong toxicity … camp chickasaw meadville paWebbZeocin ® is a formulation of phleomycin D1, a copper-chelated glycopeptide antibiotic produced by a proprietary strain Streptomyces … camp chickahominyWebb29 mars 2012 · Verification of mutant strains by drug sensitivity. (a) Phleomycin plates were incubated for 3 days at 30°C for S. cerevisiae and 5 days at 23°C for S. bayanus. first student corporate officeWebb30 nov. 2015 · Plates were photographed after 2 to 3 days. (E) The indicated strains were cultured in the presence of the indicated concentrations of phleomycin for 2 hours, diluted and plated first student east orange njWebbPhleomycin-resistant transformants are selected in Low Salt LB agar medium (yeast extract 5 g/L, Tryptone 10 g/L, NaCl 5 g/L, Agar 15 g/L, pH7.5) supplemented with 5 … first student fresno caWebbSerial dilutions of each strain were plated onto YEPD, MMS plates (0.003% and 0.004%), and phleomycin plates (3 g/ml and 6 g/ml). To measure UV sensitivity, ... first student grandview moWebbPhleomycin-resistant transformants are selected with 10-50 µg/ml of phleomycin in the regeneration medium, depending on the sensitivity of the host strain. Selectivity can be … first student first group