Phosphinothricin acetyltransferase抗性
Web产品描述. 本试剂盒提供了一种简单、方便的从培养细胞或新鲜组织中抽提核蛋白与浆蛋白的方法。. 约90分钟就可以完成培养细胞的核蛋白与浆蛋白的分离。. 抽提得到的蛋白为非变性、有活性的,可以用于Western、EMSA、footprinting、报告基因检测以及酶活力测定等 ... WebSep 12, 2014 · 河北农业大学硕士学位论文抗虫基因植物表达载体构建、遗传转化及表达研究姓名:****请学位级别:硕士专业:林木遗传育种指导教师:**生;周怀军;肖凯2009-06-07本研究在王彦平构建的植物表达载体基础上构建Bt抗虫基因表达载体,并转化烟草,对Bt基因表达水平进行检测。
Phosphinothricin acetyltransferase抗性
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WebAbstract. Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known … WebJul 1, 1990 · Integration of bar and activity of the enzyme phosphinothricin acetyltransferase (PAT) encoded by bar were confirmed in all bialaphos-resistant callus lines. Fertile transformed maize plants (R0) were regenerated, and of 53 progeny (R1) tested, 29 had PAT activity. All PAT-positive progeny analyzed contained bar.
WebDec 5, 2024 · Phosphinothricin acetyltransferase ( pat) gene confers resistance to glufosinate by transforming this herbicide into N-acetyl-l-glufosinate (NAG). The pat gene was inserted in six maize hybrids (Herculex, Agrisure TL, Herculex Yieldgard, Leptra, Viptera 3, Power Core) as a selectable marker, and its expression was evaluated by qPCR in ... WebMay 26, 2024 · PAT (phosphinothricin acetyltransferase) expression condition were varied to obtain high yield, and transformed E. coli was examined in various condition such as temperature, IPTG concentration and culturing time. IPTG concentration of 0, 0.5 and 1 mM concentration were used at 18 °C, 25 °C and 37 °C. The best condition for expression of ...
WebTransgenic plant varieties, which are tolerant to glufosinate-ammonium, were developed. The herbicide tolerance is based upon the presence of either the bar or the pat gene, … WebSep 15, 2014 · 菰抗病候选基因筛选及其转基因水稻白叶枯病抗性分析,水稻白叶枯病,白叶枯病,水稻白叶枯病防治,qq三国抗性表,qq三国抗性,dnf巨龙抗性,巨龙抗性,魔力宝贝抗性哪里学,抗性淀粉 ... bar基 因编码对除草剂PPT(Phosphinothricin)和双丙氨磷(Bialaphos)的抗性,适合 用作 ...
Webphosphinothricin N-acetyltransferase activity Source:UniProtKB-EC. Biological Process: response to antibiotic Source:UniProtKB-KW. Biological Process: response to herbicide Source:UniProtKB-KW. Expand table. Complete GO annotation on QuickGO . Keywords. Molecular function. #Acyltransferase; #Transferase;
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