Phospho-akt1/2/3 ser473 cellular kit
WebDec 16, 2009 · PIP3 recruits protein dependent kinase 1 (PDK1) and AKT, also known as protein kinase B (PKB), to the plasma membrane where AKT is phosphorylated at Thr308 by PDK1 and at Ser473 by mTOR complex 2 (TORC2) . AKT1 can also be phosphorylated at Ser473 by DNA-PK in the nucleus . AKT occupies a key regulatory node in the PI3K … WebSep 15, 2024 · The protein concentration of the supernatants was measured by BCS TM protein assay kit according to manufacturer’s ... The knockout of STK24 expression did …
Phospho-akt1/2/3 ser473 cellular kit
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WebApr 12, 2024 · Sex related differences have been observed in the progression of Alzheimer’s disease (AD) [1] and the prevalence of AD is greater in women than men in European and American patients [2, 3 ... WebAkt1/2 kinase inhibitor indeed decreased pAkt in all four cell lines , while total Akt levels were not affected by the treatment. To determine whether induction of apoptosis caused the decreased cell viability induced by the Akt1/2 kinase inhibitor, we measured the activity of caspase 3/7 in the treated cells by Caspase-Glo® assay.
WebIn contrast to previous studies suggesting the impact of Ser473 and Ser824 phosphorylation on TRIM28 function, examined phospho-mutants and phospho-mimetics did not affect …
WebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an oncogene playing a key role in controlling apoptosis, cell proliferation, transcription, cell migration, and glucose metabolism. WebPhospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when …
WebThe protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents Product specifications Designed with signal performance in mind, the kit offers a tailored standard curve ideally suited to the study of Gi coupled GPCRs. Introduction to product validation
WebThis HTRF kit is designed to monitor AKT1 phosphorylation on Ser473 as a readout of Pi3K pathway ... Learn More Phospho-AKT1/2/3 (Ser473) cellular kit chip and dale free svgWebThe phospho-AKT1 (Ser473) kit is designed for robust quantification of AKT1 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), Akt is an oncogene … chip and dale garfieldWebMyricetin ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-β signaling via targeting HSP90β chip and dale giftsWebThe Advanced Phospho-ERK assay kits are designed for the robust and highly sensitive quantification of ERK phosphorylation on Thr202/Tyr204 as an MAPK pathway readout. The simple mix-and-read protocol eliminates all wash steps for faster analysis and high quality output. The Total-ERK1/2 assay is compatible with the buffers from the Phospho or ... grant county township mapWebThe FastScan™ Phospho-Akt (Ser473) ELISA Kit detects endogenous levels of Akt when phosphorylated at Ser473 as shown in Figure 1. This kit detects proteins from the … grant county title mnWebPhospho-Akt (Ser473) Rabbit mAb has been coated on the microwells. After incubation with cell lysates, phospho-Akt (Ser473) protein is captured by the coated antibody. Following extensive washing, Akt1 Mouse Antibody is added to detect the captured phospho-Akt1 (Ser473) protein. grant county title moses lakeWebUp-regulation of O-GlcNAcylation increases Akt1 phosphorylation at Ser473. The 8305C cells were treated with 5 μM Thia-G or were transfected with 500 ng OGT plasmid, and then cell lysates were resolved by SDS-PAGE, and phospho-Akt1 (Ser473) or Akt1 were determined with specific antibodies (Figure 4A and B). An increase in Ser473 ... chip and dale garden statues