Phospho ampk antibody cst
WebAMPK is a heterotrimer consisting of a catalytic α-subunit and two regulatory subunits, β and γ. Each subunit exists as multiple isoforms (α 1, α 2, β 1, β 2, γ 1, γ 2, and γ 3 ), giving 12 different possible combinations of holoenzyme with different tissue distribution and subcellular localization ( 2 , 3 ). WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse …
Phospho ampk antibody cst
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Web使用 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb(上)或 AMPKα Antibody #2532(下),对未经处理或经寡霉素处理 (0.5 µM) 的 C2C12 细胞提取物进行蛋白质印迹分析。 使用 Phospho-AMPKα (T172) (40H9) Rabbit mAb,对对照(左)或经苯乙双胍处理(右)的石蜡包埋的 NCI-H228 细胞沉淀物进行免疫组织化学分析。 使用 Phospho-AMPKα (Thr172) … WebAMP-activated protein kinase (AMPK) is a key cellular energy sensor and regulator of metabolic homeostasis. Although it is best known for its effects on carbohydrate and lipid …
WebMonoclonal Antibody for studying Thr-P. Cited in 2 publications. Highly specific and rigorously validated in-house, Phospho-Threonine (42H4) Mouse Monoclonal Antibody (CST #5005) is ready to ship. WebApr 11, 2024 · We first found that phospho-AMPK signal widely overlapped with ... Samples were then incubated with 1:200 primary phosphorylated AMPK (pAMPK) antibody in 1.5% goat serum and 1% BSA in PBST at 4°C overnight. ... pERK (1:1000 for Western blotting and 1:2000 for IHC; Cell Signaling Technology, 4370S, lot 12, rabbit), tERK (1:1000; Cell …
WebAll lanes : Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) at 1/500 dilution Lane 1 : HUVEC cell extract untreated Lane 2 : HUVEC cell extract treated with UV Predicted band size: 64 kDa. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) WebPolyclonal Antibody for studying AMPK-alpha1 (Ser496) phosphate. Cited in 30 publications. Validated for Western Blotting. Highly specific and …
Webantibody microarray Protein Expression & Phosphorylation Profiling Learn More Cell Signaling Phospho Antibody Array 16 Signaling Pathways PI3K/AKT AMPK Apoptosis Autophagy Cell Cycle ErbB Focal Adhesion …
WebAMP-activated protein kinase (AMPK) and mechanistic target of rapamycin complex 1 (mTORC1) are meta- bolic kinases that co-ordinate nutrient supply with cell growth. AMPK negatively regulates mTORC1, and mTORC1 reciprocally phosphorylates S345/7 in both AMPK a-isoforms. northeast health system incWebJun 23, 2024 · Specifically, metformin can activate AMP-activated protein kinase (AMPK) to inactivate mammalian target of rapamycin (mTOR), change intracellular reactive oxygen species (ROS) levels, and inhibit mitochondrial functions [ 4 ]. Metformin has also been shown to regulate iron homeostasis in cells, which may be linked to cell metabolism [ 5 ]. northeast health systems tahlequahWebThe Phospho-AMPKalpha (Thr172) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to PRKAA1, and PRKAA2. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-AMPKalpha (Thr172) Antibody has been validated for the following applications: Western Blot. how to return amazon items to kohl\u0027s storeWebWestern blot analysis of Phospho-IRE1 alpha (Ser724) in HeLa cells. Samples were incubated in Phospho-IRE1 alpha (Ser724) polyclonal antibody (Product # PA1-16927) using a dilution of 2.0 µg/mL followed by an anti-rabbit HRP secondary antibody. Treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. northeast health services metrowestWebJul 1, 2024 · Adenosine 5′-monophosphate (AMP)–activated protein kinase (AMPK), a critical cellular energy sensor that increases life span in multiple species [reviewed in ()], is one of the factors required for the enhanced longevity caused by inhibition of mitochondrial respiration in C. elegans (5–7).AMPK is activated by an elevated AMP/adenosine 5′ … how to return amazon items in personWebPhospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen … how to return amazon packageWebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material. northeast hearing and speech maine