Phosphotyrosine antibody
WebAnti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is validated for use in IC, IH, IP, WB and is … WebPrimary Anti-Phospho - (Ser/Thr) antibody (ab117253) Primary Anti-Phosphotyrosine antibody [EPR16871] (ab179530) View more associated products Overview Product name …
Phosphotyrosine antibody
Did you know?
WebNov 27, 2024 · Phosphotyrosine Antibody (PY20) is a mouse monoclonal IgG 2b κ, cited in 1,334 publications, provided at 200 µg/ml. raised against p-Tyr. Phosphotyrosine Antibody (PY20) is recommended for detection of … WebThe PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to …
WebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved sensitivity and … WebImmunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Phosphotyrosine antibody [PY20] (ab197508) ab197508 staining Phosphotyrosine in PC12 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h.
WebApplication. Anti-Phosphoserine Antibody detects level of Phosphoserine & has been published & validated for use in ELISA, IH, IP & WB. 10-20 μg anti-pS/mg protein was used from a previous lot. Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of ... WebPhospho-Tyrosine (4G10) Mouse mAb recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides …
WebAnti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free See all Phosphotyrosine primary antibodies Description Rabbit monoclonal [EPR16871] to Phosphotyrosine - BSA and Azide free Host species Rabbit Tested applications Suitable for: Flow Cyt (Intra), IP, Dot blot, ELISA, ICC/IF, WB more details Species reactivity
WebFull length native protein (purified) corresponding to Phosphotyrosine. Positive control This antibody gave a positive signal in NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate. General notes This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M). shank of shoeWebThe development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. shank of the ringWebJan 12, 2012 · (a) Detection of biotinylated 4G10 (anti-Phosphotyrosine) antibody using streptavidin conjugated 655 nm QDs in a triton permeabilized embryo. Specific staining can be seen at the cell-cell boundaries of the ectodermal cells of the fin (upper part of the image) but not in the deep tissues (somites at the bottom part of the image). polymersan marine boardWebMouse anti Phosphotyrosine antibody, clone PY20 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the … shank of the bootWebTechnical Information. Immunogen. Phosphotyrosine-BSA conjugate. Clone Designation. RM111. Formulation(Request formulation change) 100 μg of protein A affinity purified antibody from an animal origin–free culture supernatant. Storage Buffer. 50% glycerol/PBS with 1% BSA and 0.09% sodium azide. shank of the rivetWebPhosphotyrosine antibody specifically binds to phosphotyrosine residues. It is a valuablel tool to analyze tyrosine phosphorylation and monitor the activity of tyrosine kinase in high throughput drug discovery. THE™ Phosphotyrosine Antibody (E10) plus recognizes a broadspectrum of phosphotyrosine-containing proteins from numerous species ... polymers and coatings short courseWebFeb 13, 2024 · Phosphotyrosine Antibodies Antibodies that detect Phosphotyrosine can be used in several scientific applications, including Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. These antibodies target … shank of omachie