Slowfade diamond antifade mountant

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August undefined, 2024

WebbProLong Diamond Antifade Mountant with DAPI provides first-class photobleach protection for Alexa Fluor fluorescent dyes and infrared proteins with nuclear DNA … WebbFluoromount has a RI of 1.40 while prolong antifade has a refactive index of 1.52. You should match the RI of the mounting solution with the immersion oil. The RI of the oil and cover slip are...

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WebbSlowFade™ Glass Antifade Mountant features a 1.52 refractive index, which is similar to that of glass coverslips, compatible immersion oil, and oil- immersion microscope … Webb2 sep. 2024 · Cells were washed three more times with PBST and mounted on slides using SlowFade Diamond Antifade Mountant (Thermofisher Scientific, Cat. #S36972). After allowing to set for 24 hr, cover-slips were sealed in place using clear nail polish. Images were captured using a Zeiss LSM 880 confocal microscope and processed using ImageJ … how is epeat funded

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WebbAfter final washing in PBS, slides were closed in SlowFade™ Diamond Antifade Mountant with 4-6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific). Sections were imaged by confocal microscopy using a Zeiss Laser Scanning Inverted Microscope LSM-700 equipped with 40X/1.3 Oil NA objective and Black Zen software (Carl Zeiss) at the Center … WebbSlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It provides … WebbFor mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft … how is epdm rubber made

ProLong™ Gold Antifade Mountant with DAPI（试剂）_10 mL

SlowFade™ Gold Antifade Mountant - Thermo Fisher Scientific

Webb11 maj 2024 · To determine whether Fgfr2 is required for AT2 cell homeostasis in the adult lung, we inactivated Fgfr2 in AT2 cells and lineage-traced these cells in adult mice to assess lung morphometry and AT2 cell fate at three time points: 1 … highland games gurtenWebbProLong Diamond Antifade Mountant with DAPI comes mixed and ready-to-use in a 2 mL dropper bottle or 10 mL bottle. When bound to DNA, DAPI can be detected using a traditional DAPI filter under UV light with an … highland games guteneck

"WebbNuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. " - Slowfade diamond antifade mountant

Slowfade diamond antifade mountant

SlowFade Antifade Mountants User Guide (Pub.No. MAN0018916 …

WebbSamples were then washed 3x in distilled water, mounted with SlowFade Diamond Antifade mounting medium with DAPI (ThermoFisher Scientific), and imaged using an SP8 Leica laser scanning confocal microscope (Leica Microsystems Inc, Buffalo Grove, IL). Statistical analyses Data statistics and bioinformatics analyses were performed using R ... WebbSlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It provides …

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WebbProLong Diamond Antifade Mountant with DAPI provides first-class photobleach protection for Alexa Fluor fluorescent dyes and infrared proteins with nuclear DNA counterstain (DAPI) within and mountant. Webb12 apr. 2024 · Coverslips were washed, stained, and mounted using SlowFade™ Diamond Antifade Mountant with DAPI (Thermo Fisher Scientific). Cells were visualised using confocal laser scanning microscopy (Zeiss LSM 800 Airyscan confocal microscope) with × 63 oil immersion objectives. Image analysis was performed using ZEN Blue and ImageJ …

WebblowFade® Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. SlowFade® … WebbSlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes …

WebbSlowFade Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle, with or without DAPI. Just apply a drop to the sample, add a coverslip, and image. When the coverslip edges are sealed with an appropriate coverslip sealant, samples can be stored for days to weeks. If long term storage is desired with a refractive ... WebbProLong™ Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. ProLong™ …

WebbFor superior antifade protection of fluorescent proteins and fluorescent dyes, SlowFade ™ Diamond Antifade Mountant is recommended. Available with or without DAPI nuclear stain. Protects dyes from fading during imaging Ready-to-use liquid, ideal for immediate sample viewing Mounted samples are stable for weeks

Webb14 feb. 2024 · Tissues were washed three more times and stained with 1X DAPI (Cat# 62248; Thermo Fisher) in TBS for 10 min and mounted on microscope slides with cover slips and SlowFade TM Gold Antifade Mountant media (Cat# S36937; Invitrogen). highland games heavy athletics eventsWebbSlowFade Diamond Antifade Mountant is a premium soft-setting mounting medium. Unlike ProLong mountants, the SlowFade mountant contains no polymer and is instead a … highland games hammer throwWebbSlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It provides superior antifade protection across the entire visual and IR spectrums. highland games helotesWebb2 juni 2024 · CD28 is a major co-stimulatory effector in immune systems constitutively expressed on naive T cells (Riley and June, 2005 ), which enhances T cell receptor (TCR)-induced signals ( Michel et al., 2001) and is involved in T cell metabolism, growth, and differentiation ( Sharpe and Freeman, 2002). how is epf calculated in malaysiaWebbFor mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant. ProLong Gold Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. highland games heavy eventsWebbSlowFade light ® Diamond Antifade Mountant with DAPI 5 × 2 mL 10 mL S36964 S36973 Refractive index of mountant: 1.42 * Product may also be stored at ≤−20°C. When stored … highland games hammerwerfenWebbFor superior antifade protection of fluorescent proteins and fluorescent dyes in cultured cells or thin tissue slices, SlowFade Diamond Antifade Mountant is recommended. For high resolution or to image thicker tissue or 3D cell culture with a focal depth of 0–500 µm, try SlowFade Glass Antifade Mountant. how is ephesus pronounced